Isoelectric Point vs Izzy Statistics Pro

Isoelectric Point (IEP) helps to estimate the charge of macromolecule (proteins, peptides, amino acids, DNA, etc.), consisting of basic and acidic residues as a function of pH value of solution. The application graphically represents the charge fluctuation over the whole range of pH values and finds out the isoelectric point value. The application can extract the protein sequence (chain by chain) from FASTA files. Layout of "IEP".  The sequence of polymer molecule can be typed into the “Sequence” field in the single letter format (YHRK…). Alternatively, it can be uploaded from the PDB-styled FASTA file (for polypeptides) located in "Documents/IEP" folder. Pressing “Load” bar button launches file browser that lets the user to choose the FASTA file. Simple press on 'Chain' button launches chain picker dialog if multiple chains are defined in FASTA file. If peptide is analysed, then tapping “Analyse” button, calculates the molar weight, counts and fills in the number of recognized acidic and basic residues into the appropriate cells in the amino acid info table. Cells for acidic residues are coloured red and for basic are blue. Each row provides three cells: 1. compound (amino acid) name or acronym. 2. pKa value (user defined), default values are provided. 3. Number of the particular residues in the macromolecule. The “-C” and “-N” cells represent standard C- and N-terminus respectively. If peptide has been terminally modified, then these fields data must be changed accordingly. The “Calculate” button initiates the analysis of the table data and outputs the IEP value. Important! Table data can be fully customized after or without any sequence analysis! IEP calculation uses table values, whatever their origin is . Long press on 'Reset' button removes amino acid data from table if the analysed molecule is not based on amino acid sequence or alternatively brings it back if peptide is analysed. Short Tap on reset button simply resets data in table. Application provides data for most common acidic and basic amino acid residues, but every cell can be updated! Sequence analysis reverts the table back to its original state. Important points: Special attention should be paid for number of -C and -N termini and their pKa values, as they depend on functional derivaterization and number of chains being analysed. If user updates any of the data cells, the program will not start to plot until user press calculate button and only if the update is accepted. The cells are colored according to the data input allowed (unchangeable): red for acidic and blue for basic function. However both acidic and basic residue chemical groups are usually provided with pKa values. In case that pKb value is provided, it can be easily converted to pKa using: pKa=pKw-pKb or pKa=14-pKb (at 25°C). User should check what the primary chemical property of the residue is: the proton donor, gaining negative charge (acidic) or proton acceptor, gaining positive charge (basic). Unrecognized amino acid residue letters are skipped, but can be added manually to the available cells. The empty cell sets are available for custom input. The pKa values can be changed in table to fit the desired model. The application assumes no influence between residues. The application assigns dot as a decimal separator.

The most complete statistical tool without advertising. With an intuitive and simple interface that will facilitate your statistical calculations wherever you are. It doesn't matter if you are a student or a professional, it is just as functional. Enter the numbers of a sample or a population separated by a space, select if the data belongs to a sample or a population, touch the calculate button and you will obtain the result of the following equations: - Ordered numbers - Total numbers - Arithmetic Mean - Median - Mode - Quartiles - Interquartile range - Range - Average deviation - Variance - Standard Deviation - Coefficient of Variation - Typical Values - Atypical Values - Extreme Atypical Values You can also create, share and get the histogram properties of the data: - Histogram - Lowest and highest data - Distribution Range - Total numbers - Classes Number - Classes Range - Classes Besides you can: - Save the data for future use and not have to enter it every time you need it - Update the data in case you need to add, modify or delete any - Delete the data when you think you no longer need it Do not forget to leave your comment and rating, as well as share the app with your friends. This helps us a lot as we can reach more people. For any question, suggestion, doubt or to report an error, send us an email. We are constantly growing to offer you the best possible app and experience.