Microscope Resolution vs Renal Transplant Pathology

Characterize your microscope objective lenses, see how well matched they are to your microscope's image acquisition system. The App supports both transmitted light objectives and fluorescence objectives. Input the magnification, immersion medium (air, oil, glycerol or silicone oil), NA (numerical aperture), condenser NA and wavelength of light (lambda), to calculate the lateral resolution, axial resolution and brightness of the objective. Characterize your acquisition system (camera pixel size, camera binning and additional magnification) to check if you're sampling at Nyquist frequency. Each of your objectives can be saved and easily restored, and shared with other microscopists via e-mail, MMS, Linked-In or even Facebook. The equations used for the calculations can be displayed. The sources of equations are referenced in the "Information" section. * Theoretical Resolution is calculated according Ernst Abbe's famous equation (Abbe 1873) * Actual Resolution is calculated according to ‘The Rayleigh Criterion’ * Axial Resolution of fluorescence objectives is calculated according to Inoue's chapter in Jim Pawley's “Handbook of Biological Confocal Microscopy” (3rd edition 2006) * Camera pixel size can be loaded from the list of cameras, if your camera is not in the list, you can enter it manually. * Select the "share" feature to share all output to e-mail, MMS or Linked-In * It doesn't matter if your microscope was made by Zeiss, Evos, Leica, Olympus, Nikon, Motic, or Meiji Techno, the principles are identical and you will find the app useful. * The list of cameras in the Camera dialog includes those made by Andor, Hamamatsu, Photometrics, PCO, QImaging, Leica, Lumenera, Olympus, Nikon, Motic and Zeiss.

Volume 7 in the Series: The Johns Hopkins Atlases of Pathology AUTHORS: Serena M. Bagnasco and Lorraine C. Racusen SERIES EDITORS: Toby C. Cornish, Norman J. Barker, and Ralph H. Hruban The Johns Hopkins Atlas of Renal Transplant Pathology is the seventh teaching app in our series from the Johns Hopkins University Department of Pathology. This app is designed to teach residents, fellows, and practicing pathologists the basic pathologic lesions in the kidney allograft. The diagnostic entities covered by the Atlas are not limited to rejection but also include recurrent glomerulopathies, infections and other diseases that may affect transplanted kidneys. The diagnostic approach to rejection follows the Banff criteria in the latest version (2017 at the time of this writing). The Banff definitions and rules, Banff scoring system, and Banff classification of rejection are available in text format for easy consultation. The images collected in this Atlas include high resolution examples of light microscopy, immunofluorescence, immunohistochemistry and electron microscopy features of individual diseases. Annotations with comments and references to definition, scoring and classification categories are also included for each image. Three teaching algorithms are provided for navigating through the combination of features leading to diagnosis of specific types of rejection or other conditions unrelated to rejection. These algorithms cover: (1) T-cell mediated rejection, (2) antibody-mediated rejection and (3) other tubular and interstitial pathologic changes. A set of representative images, each with multiple choice quiz can be used for self-test. We welcome your feedback. Please e-mail Dr. Hruban at rhruban@jhmi.edu. If you find an error, please let us know so we can correct it.