Microscope Resolution vs Thorlabs Toolkit

Characterize your microscope objective lenses, see how well matched they are to your microscope's image acquisition system. The App supports both transmitted light objectives and fluorescence objectives. Input the magnification, immersion medium (air, oil, glycerol or silicone oil), NA (numerical aperture), condenser NA and wavelength of light (lambda), to calculate the lateral resolution, axial resolution and brightness of the objective. Characterize your acquisition system (camera pixel size, camera binning and additional magnification) to check if you're sampling at Nyquist frequency. Each of your objectives can be saved and easily restored, and shared with other microscopists via e-mail, MMS, Linked-In or even Facebook. The equations used for the calculations can be displayed. The sources of equations are referenced in the "Information" section. * Theoretical Resolution is calculated according Ernst Abbe's famous equation (Abbe 1873) * Actual Resolution is calculated according to ‘The Rayleigh Criterion’ * Axial Resolution of fluorescence objectives is calculated according to Inoue's chapter in Jim Pawley's “Handbook of Biological Confocal Microscopy” (3rd edition 2006) * Camera pixel size can be loaded from the list of cameras, if your camera is not in the list, you can enter it manually. * Select the "share" feature to share all output to e-mail, MMS or Linked-In * It doesn't matter if your microscope was made by Zeiss, Evos, Leica, Olympus, Nikon, Motic, or Meiji Techno, the principles are identical and you will find the app useful. * The list of cameras in the Camera dialog includes those made by Andor, Hamamatsu, Photometrics, PCO, QImaging, Leica, Lumenera, Olympus, Nikon, Motic and Zeiss.

Get convenient access to photonics calculators, equations, and other information and resources when you download this free app! This application is being continually updated to offer new types of resources, as well as to expand existing categories, so be sure to check for updates. The current version of the app includes a collection of calculators that engineers and technicians at Thorlabs Lens Systems and elsewhere have used on the floor for over 15 years. Each calculator page includes the equations used in the calculations, as well as usage notes and information about assumptions included in the equations. Additional resources include brief summaries of ray tracing, lens theory, Snell’s Law, manufacturing tolerances, and evaluation of system resolution using the modulation transfer function (MTF). History Thorlabs Toolkit builds on the excellent foundation provided by the JML Optical Calculator. When JML Optical joined Thorlabs’ family as Thorlabs Lens Systems, it provided a wonderful opportunity to expand the app to share knowledge collected from across all of Thorlabs.